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mitomycin c inactivated human foreskin fibroblasts  (ATCC)


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    ATCC mitomycin c inactivated human foreskin fibroblasts
    Mitomycin C Inactivated Human Foreskin Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitomycin c inactivated human foreskin fibroblasts/product/ATCC
    Average 96 stars, based on 377 article reviews
    mitomycin c inactivated human foreskin fibroblasts - by Bioz Stars, 2026-03
    96/100 stars

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    a ) Conditioned media from either murine (MEFs) or human <t>(HS27)</t> embryonic <t>fibroblast</t> cells was immuno-depleted of NM23-H1. Western blot confirmed that the depleted media no longer contains NM23-H1. b ) NM23-H1 was captured by affinity chromatography, eluted and its concentration quantified by the optical density at 280nm. Human conditioned media contains 5-times more NM23-H1 than murine conditioned media. Human ES cells on Matrigel were cultured with: c, d ) NM23-H1-depleted media, e, f ) bFGF plus complete conditioned media. NM23-H1-depleted conditioned media induced differentiation ( c, d ).
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    a ) Conditioned media from either murine (MEFs) or human (HS27) embryonic fibroblast cells was immuno-depleted of NM23-H1. Western blot confirmed that the depleted media no longer contains NM23-H1. b ) NM23-H1 was captured by affinity chromatography, eluted and its concentration quantified by the optical density at 280nm. Human conditioned media contains 5-times more NM23-H1 than murine conditioned media. Human ES cells on Matrigel were cultured with: c, d ) NM23-H1-depleted media, e, f ) bFGF plus complete conditioned media. NM23-H1-depleted conditioned media induced differentiation ( c, d ).

    Journal: PLoS ONE

    Article Title: MUC1* Ligand, NM23-H1, Is a Novel Growth Factor That Maintains Human Stem Cells in a More Naïve State

    doi: 10.1371/journal.pone.0058601

    Figure Lengend Snippet: a ) Conditioned media from either murine (MEFs) or human (HS27) embryonic fibroblast cells was immuno-depleted of NM23-H1. Western blot confirmed that the depleted media no longer contains NM23-H1. b ) NM23-H1 was captured by affinity chromatography, eluted and its concentration quantified by the optical density at 280nm. Human conditioned media contains 5-times more NM23-H1 than murine conditioned media. Human ES cells on Matrigel were cultured with: c, d ) NM23-H1-depleted media, e, f ) bFGF plus complete conditioned media. NM23-H1-depleted conditioned media induced differentiation ( c, d ).

    Article Snippet: Human ES cells, H9 (WiCell), BGO1V/hOG cells (Life Technologies), human iPS cells, FTD19 clone 42 and ESIMR90 clone 4, (Kosik Lab) were cultured at 37°C and 5% CO 2 on mitomycin-C inactivated Hs27 human foreskin fibroblasts or MEFs (ATCC), Matrigel (BD Biosciences) per manufacturer's instructions, Vitronectin at (6.25 μg or 12.5 μg), or anti-MUC1* mab MN-C3 (12.5 μg/well) coated onto 6-well plates (Vita plates, ThermoFisher; or BD Falcon # 353046).

    Techniques: Western Blot, Affinity Chromatography, Concentration Assay, Cell Culture